HELEN JANE WING
CURRICULUM VITAE
CONTACT INFORMATION:
School of Life Sciences
University of Nevada, Las Vegas
4505 S. Maryland Pkwy
Las Vegas, Nevada 89154-4004
Telephone: 702 895 5382 (office) FAX: 702 895 3956
702 895 3968 (lab) Email: helen.wing@unlv.edu
EDUCATION:
1993-1997 Ph.D.
University of Birmingham, UK. Prokaryotic transcription
1990-1993 B.Sc. (Hons.) Nottingham
University, UK: Biology, specializing in microbial genetics and molecular
biology
PROFESSIONAL EXPERIENCE:
2005-present Tenure-track
Assistant Professor, School of Life Sciences,
2000-2004 Postdoctoral
Research Associate,
1997-2000 Postdoctoral Research Associate, University of Birmingham, Edgbaston, UK & University of Sheffield, UK
RESEARCH
INTERESTS:
Mechanisms of Bacterial Pathogenesis, Bacterial
Transcription and Gene Regulation, Protein Localization Studies, Anaerobiosis, Bacterial Motility and Chemotaxis,
Outer Membrane Proteases, Bacterial Resistance to Cationic Antibacterial
Peptides, Extra-host Survival of Bacterial Pathogens
Professional Societies:
· Member of the Executive Committee of the Wind River Prokaryotic Meeting.
· Member of the american Society for Microbiology (ASM)
· Treasurer and Secretary of Arizona and Southern Nevada Branch of the ASM
· Chair of the 10th Boston Bacterial Meeting
HONORS
AND AWARDS:
2009 UNLV CSUN Faculty Excellence award (Student nominated)
2008 UNLV Merit Award $3,000 (last year merit applications solicited in NV)
2007 UNLV Merit Award $2,000
2006 UNLV Merit Award $1,000
2002-2004 Postdoctoral
Fellowship from the Medical Foundation/ Charles H. Hood Foundation
Title: “Regulation of the Shigella virulence factor IcsP”
2001-2002 Postdoctoral
Fellowship from the Fund for Medical Discovery,
1995 Poster Prize presented at the Biochemical Society's Wales and West Post-graduate meeting.
1995 Chandler Poster Prize awarded at “Graduate Research Day” within the School of Biochemistry, University of Birmingham, UK.
1993 B.B.S.R.C. Post-Graduate Studentship awarded to Prof. S. Busby, University of Birmingham
1993 Graduated with Honors: University of Nottingham
1992 Wellcome Summer Research Fellowship, awarded to Dr. R. E. Sockett, University of Nottingham, UK.
FUNDING:
Federal/Extramural Grants
Current:
1 R15 AI090573-01: (reviewed by Prokaryotic Cell Molecular Biology; Impact Score: 11)
NIH: NIAID 07/01/10-06/30/13
“Understanding transcriptional silencing & anti-silencing mechanisms in Shigella” ($405,488)
Role: PI
Pending:
0649267
NSF- Research Experience for Undergraduates Regner
(PI) 03/06/07-02/28/10
“A broad view of Environmental Microbiology
at UNLV” ($254,637)
This study provide 8 summer studentships for
undergraduates to work with UNLV faculty in the field of environmental
microbiology
Role: Key Personnel
Status:
Recommended for funding
USDA-NIFA Elekonich (PI) 11/01/10-10/31/13
“New treatments for American Foulbrood; using the microbe’s biology against it” ($335,417)
Role: Co-PI
Other Co-PIs: Drs. Abel-Santos, & Amy (UNLV faculty)
Status: Under review
Complete:
0649267
NSF- Research Experience for Undergraduates Regner
(PI) 03/06/07-02/28/10
“A broad view of Environmental Microbiology
at UNLV” ($254,637)
This study provide 8 summer studentships for
undergraduates to work with UNLV faculty in the field of environmental
microbiology
Role: Key Personnel
0649267
NSF Supplement - Research Experience for
Teachers Wing (PI) 06/06/08-02/28/10
“A broad view of Environmental Microbiology
at UNLV” ($15,000 x 2)
This supplement supports a high school teacher
to work with Dr. Wing in the field of Environmental Microbiology
Role: Mentor
0649267
NSF Supplement - Research Opportunity Award Wing (PI) 06/06/09-02/28/10
“A broad view of Environmental Microbiology
at UNLV” ($17,500)
This supplement supports a professor from a
tertiary college to work with Dr. Wing in the field of Environmental
Microbiology
Role: Mentor
Unfunded:
1. National Institutes of Health: NIAID: R21 ($275,000) (resubmission)
“Exploring the roles and regulation of the Shigella protease IcsP”
Role: PI
Consultants: James Huntley National Center for Genome Research, NM; Christian Ross, Bioinformaticist UNLV
2. NSF Undergraduate Research Mentoring Program
“URM Preliminary Proposal: Research and Mentoring in molecular cell Biology and Microbiology at UNLV”
Role: Co-PI; PI: Kurt Regner
3. National Institutes of Health: NIAID: R21 ($275,000)
“Exploring the roles and regulation of the Shigella protease IcsP”
Role: PI
Consultants:
4. Request for instrumentation: ($79,122 )
Title: “Tissue culture instrumentation to further studies on gene expression in hibernators and assess Shigella virulence and primary defense mechanism in human cells.”
Department of Defense: Defense University Research Instrumentation Program (DURIP)
Role: PI
Co-Pi: Dr. van Breukelen
5. National Institutes of Health: NIAID: RFA: U01: International Collaboration in Inf. Dis. ($2,519,378.00)
Title: “Novel therapies & strategies against Shigellosis”
Role: PI
Major Foreign Collaborator: Rubhana Raqb,
Co-PI: Birgitta Agerberth, Karolinska Institutet
Collaborators: Marcia Goldberg, Massachusetts General Hospital, Gudmundur Gudmundsson, University of Iceland, Jan Andersson, Karolinska University Hospital, David Sack, ICDDR,B
State/Local/Intramural Grants
Current:
None
Pending:
2P20 RR 016464 – 05 (reviewed with Impact Score 20)
NIH/IDEA network of Biomedical Research
Excellence (INBRE) 06/01/10-05/31/11
“Roles and regulation of a Shigella protease” ($50,000)
This study investigates the roles and
regulation of an outer membrane protease found in Shigella
Role: Target Faculty
Complete:
1. IDEA network of Biomedical Research Excellence (INBRE) target faculty in NV 06/01/08-5/31/10
“Roles and regulation of a Shigella protease” ($270,000)
2. UNLV President’s Research Award
(partially funded $6,000) 07/01/07-6/30/09
“Support of graduate projects focusing on microbial interactions with plant and animal cells.” ($50,000)
Role: PI
Co-PIs: Ernesto Abel-Santos and Eduardo Robleto
3. New
Investigator Award 06/01/06-05/31/08
University of
Nevada, Las Vegas ($15,000)
“Identification
of additional roles of IcsP, a bacterial protease of the pathogen Shigella
flexneri”
This study demonstrated that IcsP does not protect Shigella from
the action of cationic antimicrobial peptides
Role: PI
Unfunded:
1. Institute for Security Studies: Summer Research Award
“Measuring the survival of bioterrorism agents in water” ($20,000)
Role: PI; Co-investigators: Debbie Soukup and Brenda Buck
2. IDEA network of Biomedical Research
Excellence (INBRE) target faculty in
“Characterization of the roles and regulation of a family of bacterial proteases during growth and differentiation in the host environment” ($270,000)
3. Department of Defense - EPSCoR
“Assessment of health risks associated with Shigella contaminated water using molecular and cellular approaches” ($698,665)
Ranked: Second tier (118/180)
4.
“Planetary protection of Mars: infectious dose” ($44,849)
Role: Co-PI; PI: Henry Sun
5. Nevada EPSCoR White paper
“
Role: Team member; PI: Alison Murray; Co-PI: Dennis Bazylinski
PUBLICATIONS:
1) Savery
NJ, Rhodius VA, Wing HJ, Busby SJW. 1995.
Transcription activation at Escherichia
coli promoters dependent on the cyclic-AMP
receptor protein – effects of binding sequences for the RNA polymerase alpha
subunit. Biochem. J. 309:
77-83.
2)
Wing HJ, Williams SM, Busby SJW.
1995. “Spacing requirements for transcription activation by Escherichia coli FNR protein.” J. Bacteriol.
177: 6704-6710.
3)
Savery N, Belyaeva T, Wing H, Busby S. 1996. Regulation of
promoters by two transcription activators: Evidence for a 'simultaneous
touching' model. Biochem. Soc. Trans. 24: 351-353.
4) Williams SM, Savery NJ, Busby SJW, Wing
HJ. 1997 Transcription activation at Class I FNR-dependent promoters:
identification of the activating surface of FNR and the corresponding contact
site in the C-terminal domain of the RNA polymerase alpha subunit. Nucl. Acid Res. 25: 4028-4034.
5) Li B, Wing
H, Lee D, Wu HC, Busby SJW. 1998. Transcription activation by Escherichia coli FNR protein:
similarities to, and differences from, the CRP paradigm. Nucl. Acid Res. 26: 2075-2081.
6) Williams
SM, Wing HJ, Busby SJW. 1998. Repression of transcription initiation by Escherichia coli FNR protein: repression
by FNR can be simple. FEMS
Microbiol. Letts, 163: 203-208.
7) Wing HJ, Green J, Guest
JR, Busby SJW. 2000. Role of activating region 1 of Escherichia coli FNR protein in transcription activation at class
II promoters. J. Biol. Chem. 275:
29061-29065.
8) Lee DJ, Wing
HJ, Savery NJ, Busby SJW. 2000. Analysis of
interactions between activating region 1 of Escherichia
coli FNR protein and the C-terminal domain of the RNA polymerase alpha
subunit: use of alanine scanning and suppression
genetics. Mol. Microbiol.
37: 1032-1040.
9) Marshall FA,
Messenger SL, Wyborn NR, Guest JR, Wing H,
Busby SJW, Green J. 2001. A novel promoter
architecture for microaerobic activation by the
anaerobic transcription factor, FNR. Mol.
Microbiol. 39: 747-753.
10) Wing HJ, Yan AW, Goldman
SR and Goldberg MB. 2004. Regulation of IcsP, the outer membrane protease of
the Shigella actin tail assembly protein IcsA, by virulence plasmid
regulators VirF and VirB. J. Bacteriol. 186:
699-705.
11) Wing HJ, Goldman SR, Ally S, Goldberg
MB. 2005. Modulation of an outer membrane protease contributes to the virulence
defect of Shigella flexneri strains carrying a mutation in
the virK
locus. Inf Immun. 73:1217-1220.
12) Castellanos M, Harrison DJ,
Smith JM, Labahn SK, Levy KM and Wing HJ. 2009. “VirB alleviates H-NS
repression of the icsP promoter in Shigella flexneri from sites over 1 kb
upstream of the transcription start site” J. Bacteriol.
191: 4047-4050
13) Hensley CT, Kamneva O, Levy KM, Labahn SK,
Africa LAA and Wing HJ. 2010. Two
promoters and two translation start sites control the expression of the Shigella flexneri outer membrane
protease IcsP. Archives of Microbiology (accepted 12/15/10)
INVITED LECTURES AND INTERNATIONAL/NATIONAL
SYMPOSIA PRESENTATIONS
·
Invited Seminar at University of Louisiana,
Lafayette. Hosted by Dr. Don Ennis. (January 21st 2010) “Roles and
Regulation of a Shigella protease,
IcsP”
·
Invited Seminar at Touro University, Nevada. Hosted by Dr. Mahboob
Qureshi. (February 3rd 2010) “Roles and
Regulation of a Shigella protease,
IcsP”
·
Invited Seminar at University of
Ohio. Hosted by Dr. Erin Murphy. (October 23rd, 2009) “Roles and
Regulation of a Shigella protease,
IcsP”
·
Gordon Conference on Bacterial Pathogenesis and
Toxins (2008)
·
Wind River Conference on
Prokaryotic Biology, Estes Park, CO (June 2008)
TEACHING
Course Development
Microbiology (formerly Microbial Systems): Survey of general microbiology including microbiological prokaryotic cell structure and function, genetics, ecology, and pathogenesis. Three hours lecture, three hours laboratory; class meets twice a week.
Bacterial Pathogenesis: This course addresses the molecular mechanisms by which bacterial pathogens cause disease. Basic principles of bacterial pathogenesis will be considered before a survey of bacterial pathogens and their specific virulence factors is conducted. This course includes aspects of bacterial genetics, physiology, immunology and the cell biology of host-parasite interactions. Three hours lecture, class meets twice a week.
School of Life Sciences, UNLV (status,
enrollment, average student evaluative GPA from 0 to 4)
2010, Spring BIOL 351: Microbiology (lecture, 97, 3.56)
BIOL 492: Undergraduate Research: Nick Egan
BIOL 493: Undergraduate Seminar “Current topics in Microbiology” (seminar, 2, N.D.)
BIOL 796: Graduate Seminar “Current topics in Microbiology” (seminar, 4, N.D.)
2009, Fall BIOL 464: Bacterial Pathogenesis (lecture, 87, 3.6)
BIOL 664: Graduate Course: Bacterial Pathogenesis (lecture, N.D., 1)
BIOL 492: Undergraduate Research: Nick Egan and David Basta
2009, Spring BIOL 351: Microbiology (lecture, 3.5, 81)
BIOL 492: Undergraduate Research: Lia Africa
2008, Fall BIOL 464x: Bacterial Pathogenesis (NEW COURSE for NSHE) (lecture, 43, 3.65)
BIO 730C: Graduate Problems in Biology: Bacterial Pathogenesis (lecture, 2, 4.0)
BIOL 492:
Undergraduate Research: Maria Castellanos
2008, Spring BIOL 351: Microbiology (lecture, 69, 3.64)
BIO 730C: Graduate Problems in Biology: Bacterial Pathogenesis (lecture, 4, 4.0)
BIOL 492: Undergraduate Research: William Ingersoll and Karen Levy
2007, Fall BIOL 464x: Bacterial Pathogenesis (NEW COURSE for NSHE) (lecture, 32, 3.54)
BIO 730C: Graduate Problems in Biology: Bacterial Pathogenesis (lecture, 4, 4.0)
BIOL 492:
Undergraduate Research: William Ingersoll and Karen Levy
2007, Spring BIOL 493: Undergraduate Seminar “Current topics in Microbiology” (seminar, 4, N.D.)
BIOL 492: Undergraduate Research: Tara Sutherland
BIO 796: Graduate Seminar “Current topics in Microbiology” (seminar, 6, N.D.)
2006, Fall BIOL 464x: Bacterial Pathogenesis (NEW COURSE for NSHE) (lecture, 32, 3.54)
BIO 730C: Graduate Problems in Biology: Bacterial Pathogenesis (lecture, 4, 4.0)
BIOL 492: Undergraduate Research: Ashley Connell
2006, Spring BIOL 351X: Microbial Systems (NEW COURSE for NHSE) (lecture, 45, 2.82)
BIOL 492: Undergraduate Research: Tim Struve
2005, Fall BIOL 493: Undergraduate Seminar “Current topics in Microbiology” (seminar,4, 4.0)
BIOL 492: Undergraduate Research: Noam Moas
BIO 796: Graduate Seminar “Current topics in Microbiology” (Seminar, 6, 4.0)
2005, Summer BIO 492: Undergraduate Research: Lisa Locaynia and Julius de Leon
2005, Spring New Investigator Teaching release
TRAINEES:
Graduate Students Mentored:
Darla deStephanis (joins Fall 2010)
Krystle Pew (M.S. in progress)
Christopher Hensley (M.S. in progress)
Sara M. Kienzle (M.A.S. in progress)
Lt. Dustin Harrison (Ph.D. graduated 2010)
Eun-Hae Kim (M.S. graduated 2008)
Bilal Zaatiti (Ph.D. Left program)
Other Graduate Student Committee
Memberships:
Adelbert Villoso (M.S. School of Life Sciences, UNLV in progress)
Israel Alvarado (Ph.D. School of Life Sciences, UNLV in progress)
John Perry (Ph.D. School of Life Sciences, UNLV in progress)
Hyelee Lee (M.S. School of Chemistry, UNLV in progress)
Patricia Ringler (Ph.D. School of Life Sciences, UNLV in progress)
Mohammed Abdul Ata-Ur Rahman (Ph.D. School of Chemistry, UNLV in progress)
Holly Martin (M.S. School of Life Sciences, graduated 2010)
Andy Phui (M.S. Department of Chemistry, graduated 2010)
Randy Boyles (Ph.D. School of Life Sciences, graduated 2010)
Nicole Bond (Ph.D. School of Life Sciences, graduated 2010)
Caitlin Murphy (M.S. School of Life Sciences, graduated 2009)
Tom Welch (Internship, School of Public Health, graduated 2009)
Zadkiel Alvarez (M.S. Department of Chemistry, graduated 2009)
Linh Nguyen (Ph.D. School of Life Sciences, graduated 2009)
Katila Varivarn (M.S. School of Life Sciences, graduated 2009)
Vanja Velickovska (M.S. School of Life Sciences, graduated 2008)
Robin Miller (M.S. School of Life Sciences, graduated 2008)
Rowena Manalang (M.S. School of Life Sciences, UNLV left the program)
Ryan MacDonald (Ph.D. School of Life Sciences, UNLV left program)
Yasmin Younas (Ph.D. School of Life Sciences, UNLV left program)
Undergraduate Students:
Lisa Locaynia*, Julius de Leon*>, Noam Moas, Tim Struve, Meghann Ditsworth, Aerial Carlson, Young Park, Ashley Connell*, Jennifer Smith¨, Tara Sutherland*, William Ingersoll, Karen Levy$*, Maria Castellanos*, David Basta*, Lia Africa*, Amanda Yates¨, Monique Gomez¨, Nick Egan, Nateira Magnuson, Juan Duhart*
* Recipient of Summer INBRE Undergraduate Fellowship; > Recipient of Center for
Math and Science Education EPSCoR Undergraduate
Research Award; ¨Recipient of NSF
“Research Experience for Undergraduates” (REU) fellowship; $ Recipient of National Goldwater
Scholarship
SERVICE
Profession:
2010 NSF Panelist (reviewed 15 grants for
Molecular and Cellular Biosciences)
2010 Chair of organizing committee of Annual meeting of AZ/NV ASM branch in Las Vegas, NV
2006 – to date Treasurer/Secretary,
AZ/Southern NV branch of ASM
2009 - to date Member of Executive committee
for the Wind River Conference on Prokaryotic Biology
2009 Reviewer of manuscript for FEBS journal
2009 Judge, Graduate Research Talks and Posters at the Wind River Conference on Prokaryotic Biology, CO
2006 Co-organizer, Annual
meeting of AZ/NV ASM branch in Las Vegas, NV
2006 Judge, Graduate Research Talks and
Posters at the Wind River Conference on Prokaryotic Biology, CO
2005 Ad hoc reviewer of NSF grant
2005
Session Chair at
Wind River Conference on Prokaryotic Biology, Estes Park, CO
2005 Judge, Undergraduate &
Graduate Research Talks at
2003
Chair, 10th Boston
Bacterial Meeting
2002
Fundraiser, 9th
Boston Bacterial Meeting
System:
2009 Hosted Prof. Robin Herlands research project in Summer. Project sponsored by the NSF
2009 Mentored Clark County High School Teacher in Summer. Project sponsored by the NSF
2008 Mentored Clark County High School Teacher in Summer. Project sponsored by the NSF
2006 Petitioned Colleges in the Nevada System Higher Education to introduce the new microbiology course Biol 351X
2005 - to date Adjunct Assistant Professor at
2006 – to date McNair Program Faculty Mentor for Undergraduate Projects
2005 - to date INBRE Faculty Mentor for Undergraduate Projects
2005 - to date EPSCoR Faculty Mentor for Undergraduate Projects
University:
2008 Attended Graduate & Professional Student Association Meeting
2008 New Graduate Course approved Biol 664
2006 Interview with Erin O’Donnell, UNLV magazine
2005 Ad hoc reviewer of UNLV SITE proposals
College:
2010 Interviewed by the NWCCU accreditation agency
2008 Presented poster at NevBIO’s Award Ceremony (Harry Reid present)
2008 Attended seminars during the COS Dean Search
2006
Coordinator,
Science and Engineering Building, UNLV proposal
2005 Presenter, Association of PreHealth Professionals at UNLV
School:
2005-to date Graduate Operations Committee. Monitored all graduate students’ progress through the graduate program. Co-revised Graduate Handbook and Catalog
2006-2008 Personnel Committee. Co-revised new bylaws for the School of Life Sciences
2006 Judge, Undergraduate and Graduate talks
at BIOS Symposium
2006 Microbiology Search Committee. Resulted in the hiring of Dr. Dennis Bazyliski
2005-to date Ad
hoc committee for autoclave maintenance and safety training
Unit:
2005- to date Maintenance
of the Microbiology group website (www.unlv.edu/faculty/hjwing/microbiology.htm)
2005 Member, Ad hoc Committee Member for Integrated
Cell Biology and Microbiology Graduate Program
Reform
2005 Member,
Ad hoc Committee for Undergraduate Microbiology Curriculum reform
2005 - to date Host, 7 School Seminar
Speakers: Dr. Gudmundur Gudmundsson,
Dr. Lawrence Goodridge and Dr. Carolyn Hovde-Bohach, Dr. Doug Crabb, Dr.
Marty Roop, Dr. Erin Murphy and Dr. George Munson
Community:
2010 Invited Speaker at the Mathematics Integrated with Science using Technology (MIST) Summer Institute “Living with Radiation”. Clark County School District
2008 Invited Speaker at the Middle School Teachers Summer Workshop on “Infectious Disease”. Clark County School District
2005 Television
Interview for Channel 13 News-Expert on Bacterial Pathogenesis. Story
concerned bacterial contamination on shopping cart trolleys