Laemmli Buffer

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Regular Laemmli Buffer

0.2 mL SDS, pH to 8.5.
22g Tris Base
12g Boric acid.
add dH₂O to a total volume of 2 L, mix well.

2x Sample Buffer

12.5 mL 1.0M Tris-HCl (pH 6.8)
40 mL 10% SDS
approx. 30 mL glycerol (37.7g)
1 mL ß-mercaptoethanol
"a pinch" of bromophenol blue
Raise to 100 mL with dH₂O.

4x Laemmli Buffer *

3g Tris-HCl pH 6.8 (250 mM)
40mL 40% (v/v) Glycerol
5g 5% (p/v) SDS
5mg 0.005% (p/v) Bromophenol Blue
Store at room temperature. **

5x Sample Buffer

10% w/v SDS
10 mM dithiothreitol or β-mercaptoethanol
20 % v/v glycerol
0.2 M Tris-HCl, pH 6.8
0.05% w/v bromophenol blue

Notes

* Makes 100 mL.

** Add 10% β-mercaptoethanol just before use.