Protocols

Here you will find all the protocols used in the Andres Lab. All protocols can be viewed in HTML and PDF form. Please click on the links below to view each protocol.

Each category is alphabetically listed, with each protocol listed alphabetically under each section.

Antibody Staining

E63-1 Antibody Staining Of Salivary Glands
Farkas Immunostaining Of Salivary Glands
Golgi Immunostaining Of Salivary Glands

DNA

SURE 2 Supercompetent Cell Transformation

Protein

BCA Assay For Protein Concentration
E63-1 x HIS Purification
E63 Western Blot
ECL Western Blot

RNA

Northern Blot (non-radioactive)
Northern Blot (hybridization bags)
Northern Blot (hybridization bottles)

Miscellaneous

Laemmli Buffer
LysoTracker
Salivary Gland Extraction For Western Blot
Typhoon - HRP Antibody Detection